Selection of suitable reference lncRNAs for gene expression analysis in <i>Osmanthus fragrans</i> under abiotic stresses, hormone treatments, and metal ion treatments.

ABSTRACT:

Intoduction

Osmanthus fragrans, a well-regarded traditional flower in China, holds extensive applications in gardening, food, cosmetics, and traditional Chinese medicine. Despite its importance, research on long non-coding RNAs (lncRNAs) in O. fragrans has been constrained by the absence of reliable reference genes (RGs).

Methods

We employed five distinct algorithms, i.e., delta-Ct, NormFinder, geNorm, BestKeeper, and RefFinder, to evaluate the expression stability of 17 candidate RGs across various experimental conditions.

Results and discussion

The results indicated the most stable RG combinations under different conditions as follows: cold stress: lnc00249739 and lnc00042194; drought stress: lnc00042194 and lnc00174850; salt stress: lnc00239991 and ln

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC11790643 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature