Forensically relevant anatomical brain regions cannot be sub-differentiated by RNA expression analysis.

ABSTRACT: The contextualization of biological traces generated by severe head injuries can be beneficial for criminal investigations. Here we aimed to identify and validate mRNA candidates for a robust sub-differentiation of forensically and traumatologically relevant brain regions. To this purpose, massively parallel sequencing of whole transcriptomes in sample material taken from four different areas of the cerebral cortex (frontal, temporal, parietal, occipital lobe) was performed, followed by bioinformatical data analysis, classification, and biostatistical candidate selection. Candidates were evaluated by Multiplex-RT-PCR and capillary electrophoresis. Only a weak relative upregulation and solely for candidates expressed in the parietal lobe was observed. Two candidates with upregulation in the

SUBMITTER: Euteneuer J 

PROVIDER: S-EPMC11790677 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature