A phosphorylation-regulated NPF transporter determines salt tolerance by mediating chloride uptake in soybean plants.

ABSTRACT: Chloride (Cl^-) ions cause major damage to crops in saline soils. Understanding the key factors that influence Cl^- uptake and translocation will aid the breeding of more salt-tolerant crops. Here, using genome-wide association study and transcriptomic analysis, we identified a NITRATE TRANSPORTER 1 (NRT1)/PEPTIDE TRANSPORTER family (NPF) protein, GmNPF7.5, as the dominant gene locus influencing Cl^- homeostasis in soybean (Glycine max). A natural SNP variation resulted in two haplotypes (GmNPF7.5^HapA and GmNPF7.5^HapB), which was associated with Cl^- content. GmNPF7.5^HapA mediated Cl^- or nitrate (NO₃^-) uptake in a pH-dependent manner and exhibited higher permeability for Cl^-

SUBMITTER: Wu Y 

PROVIDER: S-EPMC11790925 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature