A multiplex method for rapidly identifying viral protease inhibitors.

ABSTRACT: With current treatments addressing only a fraction of pathogens and new viral threats constantly evolving, there is a critical need to expand our existing therapeutic arsenal. To speed the rate of discovery and better prepare against future threats, we establish a high-throughput platform capable of screening compounds against 40 diverse viral proteases simultaneously. This multiplex approach is enabled by using cellular biosensors of viral protease activity combined with DNA-barcoding technology, as well as several design innovations that increase assay sensitivity and correct for plate-to-plate variation. Among >100,000 compound-target interactions explored within our initial screen, a series of broad-acting inhibitors against coronavirus proteases were uncovered and validated through or

SUBMITTER: Hong SJ 

PROVIDER: S-EPMC11790949 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature