Oncogenic PIK3CA corrupts growth factor signaling specificity.

ABSTRACT: Technical limitations have prevented understanding of how growth factor signals are encoded in distinct activity patterns of the phosphoinositide 3-kinase (PI3K)/AKT pathway, and how this is altered by oncogenic pathway mutations. We introduce a kinetic, single-cell framework for precise calculations of PI3K-specific information transfer for different growth factors. This features live-cell imaging of PI3K/AKT activity reporters and multiplexed CyTOF measurements of PI3K/AKT and RAS/ERK signaling markers over time. Using this framework, we found that the PIK3CA^H1047R oncogene was not a simple, constitutive activator of the pathway as often presented. Dose-dependent expression of PIK3CA^H1047R in human cervical cancer and induced pluripotent stem cells corrupted the fid

SUBMITTER: Madsen RR 

PROVIDER: S-EPMC11791070 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature