Nanopore sequencing of protozoa: Decoding biological information on a string of biochemical molecules into human-readable signals.

ABSTRACT: Biological information is encoded in a sequence of biochemical molecules such as nucleic acids and amino acids, and nanopore sequencing is a long-read sequencing technology capable of directly decoding these molecules into human-readable signals. The long reads from nanopore sequencing offer the advantage of obtaining contiguous information, which is particularly beneficial for decoding complex or repetitive regions in a genome. In this study, we investigated the efficacy of nanopore sequencing in decoding biological information from distinctive genomes in metagenomic samples, which pose significant challenges for traditional short-read sequencing technologies. Specifically, we sequenced blood and fecal samples from mice infected with Trypanosoma brucei, a unicellular protozoan know

SUBMITTER: Hunter B 

PROVIDER: S-EPMC11791290 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature