High-speed centrifugation reduces immune rejection by removing bone marrow elements from fresh osteochondral allografts.

ABSTRACT:

Background

Fresh osteochondral allografts (OCAs) contain numerous immunogenic components in the subchondral bone (SB). Whether high-speed centrifugation (HSC) reduces immune rejection by removing bone marrow elements (BMEs), compared to methods without HSC, remains unknown. This study aimed to validate the efficacy and safety of HSC in reducing immune rejection by removing allogeneic BMEs.

Methods

OCAs were obtained from the femoral condyles of the stifle joint in 18 pigs. Gross observations, histological staining, weight measurements, and DNA extraction were performed to assess the effects of centrifugation speed and duration on BMEs removal in OCAs. The effect of HSC on OCAs preservation was determined in vitro using microbiological testing, live/dead cell staining, and hi

SUBMITTER: Ma Y 

PROVIDER: S-EPMC11791335 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature