Decoding <i>MexB</i> efflux pump genes: structural, molecular, and phylogenetic analysis of multidrug-resistant and extensively drug-resistant <i>Pseudomonas aeruginosa</i>.

ABSTRACT:

Objective

Emerging drug resistance in Pseudomonas aeruginosa is of great concern in clinical settings. P. aeruginosa activates its efflux-pump system in order to evade the effect of antibiotics. The current investigation aims to detect MexB genes in P. aeruginosa, their structural and molecular analysis and their impact on antimicrobial susceptibility profiling.

Methods

A total of 42 clinical specimens were aseptically collected from hospitalized patients who had underlying infections related to medical implants. Matrix-assisted laser desorption ionization-time of flight (MALDI-ToF) were used for the identification of isolates. The methods used in this study were antibiotic susceptibility profiling, minimum inhibitory concentration (MIC), polymera

SUBMITTER: Habib MB 

PROVIDER: S-EPMC11791646 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature