Single-Nucleus RNA Sequencing Reveals That Gabra6⁺ Neurons in Prefrontal Cortex Promote the Progression of PTSD After Shockwave-Induced TBI.

ABSTRACT: Shockwave-induced traumatic brain injury (TBI) results in the onset of post-traumatic stress disorder (PTSD), triggered either by the TBI itself or other stressors. However, the interplay and underlying mechanisms of how these factors synergistically induce PTSD remain inadequately elucidated. Here, mice in the TBI (induced by biological shock tube blast injury) and PTSD (induced by single prolonged stress method) groups both displayed symptoms of PTSD behaviors, with the TBI+PTSD (composite model) group exhibiting more severe manifestations. The result of snRNA-seq demonstrated a noticeable increase in the population of Gabra6⁺ neurons in the prefrontal cortex region of mice in the TBI+PTSD group. Knocking down cortical Gabra6 mitigated PTSD-related behavioral outcomes. Mechani

SUBMITTER: Fei X 

PROVIDER: S-EPMC11792052 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature