Recruitment of chromatin remodelers by XIST B-repeat region is variably dependent on HNRNPK.

ABSTRACT: X-chromosome inactivation is triggered by the long non-coding RNA XIST, whose structure is characterized by tandem repeats that modularly recruit different proteins and chromatin remodelers. Previously, we reported that the addition of the mouse PID region to a transgene with human repeat regions A, F and E (miniXIST; 5.1 kb) enabled binding of HNRNPK and also enabled the induction of silencing and recruitment of H3K27me3, UbH2A and H4K20me1, but only partially. As the 680 bp PID region enabled so many features of inactivation, we hypothesized that augmenting the PID with more mouse or human sequences rich in CCC motifs would allow us to design a short transgene which was as effective as Full XIST. Three new transgenes using the A, F and E human domains as a backbone were tested for abilit

SUBMITTER: Navarro-Cobos MJ 

PROVIDER: S-EPMC11792242 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature