HACE1 protects against myocardial ischemia-reperfusion injury via inhibition of mitochondrial fission in mice.

ABSTRACT:

Background

HECT domain and Ankyrin repeat Containing E3 ubiquitin-protein ligase 1 (HACE1) has been found to be associated with mitochondrial protection. Mitochondrial damage is a critical contributor to myocardial ischemia-reperfusion injury (I/RI). However, little is known about the role of HACE1 in the pathogenesis of myocardial I/RI.

Methods

Male C57BL6 mice with HACE1 knockout (KO) were subjected to 30 min of ischemia via ligation of the left anterior descending artery, followed by 0, 2, 6, or 24 h of reperfusion. The mice were evaluated for myocardial histopathological injury, serum troponin I (cTnI) levels, oxidative stress injury, apoptosis and cardiac function. Prior to ischemia, Mdivi-1(1.2 mg/kg) or vehicle was administered.

Results

The study revealed that

SUBMITTER: Liu BX 

PROVIDER: S-EPMC11792395 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature