Molecular identification and antibiotic clearance of <i>Mycoplasma arginini</i> and <i>Mycoplasma orale</i> from cell cultures infected with <i>Rickettsia</i> or <i>Ehrlichia</i> species.

ABSTRACT: Mycoplasma (Class: Mollicutes) contamination in cell cultures is a universal concern for research laboratories. Some estimates report contamination in up to 35% of continuous cell lines. Various commercial antibiotic treatments can successfully decontaminate clean cell lines in vitro; however, in vitro decontamination of bacterial cultures remains challenging. Intracellular bacteria like those in the genera Rickettsia and Ehrlichia require cell culture for primary isolation and propagation and are thus vulnerable to contamination with mycoplasmas. Some analyses have reported successful antibiotic clearance of contaminating mycoplasmas in Rickettsia cultures; however, many of these studies do not identify the contaminating mycoplasma species and often incl

SUBMITTER: Allerdice MEJ 

PROVIDER: S-EPMC11792515 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature