Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activity.

ABSTRACT:

Background

Angiotensin-Converting Enzyme 2 (ACE2) is a crucial peptidase in human peptide hormone signaling, catalyzing the conversion of Angiotensin-II to Angiotensin-(1-7), which activates the Mas receptor and elicits vasodilation, increased blood flow, reduced inflammation, and decreased pathological tissue remodeling. This study leverages protein engineering to enhance ACE2's therapeutic potential for treating conditions such as respiratory viral infections, acute respiratory distress syndrome, and diabetes. Surrogate substrates used in traditional high-throughput screening methods for peptidases often fail to accurately mimic native substrates, leading to less effective enzyme variants. Here, we developed an ultra-high-throughput droplet microfluidic platform to screen peptida

SUBMITTER: Okal EF 

PROVIDER: S-EPMC11792573 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature