Exploration of different quantitative polymerase chain reaction-based genotyping methods to distinguish Apcmin/+ mice from wildtype mice.

ABSTRACT: Various molecular methods have been established for genotyping single-nucleotide variants (SNVs). However, despite the widespread availability of quantitative polymerase chain reaction (qPCR) instruments in biomedical laboratories, the lack of professional analytical tools impedes the application of qPCR in genotyping. Apcmin/+ mice, which harbour a germline Apc mutation (g.2549T>A) associated with multiple intestinal neoplasms, are extensively employed in colorectal cancer research. In this study, we used Apc as a model and assessed the feasibility of different qPCR-based methods for SNV genotyping, considering approaches with and without genotyping analytical tools. We initially employed allele-specific PCR followed by electrophoresis to determine the genotypes of Apc in tail tissues fro

SUBMITTER: Sun Y 

PROVIDER: S-EPMC11793731 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature