Silicone Loss During Histological Preparation of Breast Implant Tissue From Capsular Contracture, Quantified by Stimulated Raman Scattering Microscopy.

ABSTRACT: Breast augmentations, commonly performed for aesthetic or medical reasons, often use silicone (polydimethylsiloxane [PDMS]) implants. Some patients develop complications like capsular contracture, where scar tissue forms around the implant. Previously, we used stimulated Raman scattering (SRS) microscopy to detect and quantify silicone in stained capsule tissue, finding a correlation between silicone amount and contracture severity. However, we suspected silicone loss during histological preparation, which includes multiple steps like formalin fixation and paraffin embedding. In this study, we assessed silicone loss by comparing adjacent tissue samples from the same capsule: one prepared conventionally and the other snap-frozen. SRS microscopy revealed that snap-frozen samples had roughly

SUBMITTER: Schmidt RW 

PROVIDER: S-EPMC11793946 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature