PCR-based detection of Botryosphaeria canker pathogens in fig trees.

ABSTRACT: Canker and dieback, caused by members of the Botryosphaeriaceae family, pose significant threats to plant productivity, food security, and natural ecosystems, particularly in economically important woody crops including fig trees. Detecting and identifying these pathogens is challenging due to their latent infections and the presence of multiple pathogens within the same host. In our study, we developed a PCR assay using three carefully selected primer pairs based on sequence differences in two protein-coding genes, β-tubulin and RNA polymerase II. The species-specific primers TUB-Bd1 (specific for Botryosphaeria dothidea), TUB-Np1 (specific for Neofusicoccum parvum), and RPB-Nd2 (specific for Neoscytalidium dimidiatum) effectively amplified target gene sequences in pure cultures and infec

SUBMITTER: Ghaedi M 

PROVIDER: S-EPMC11794561 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature