The Sarcoplasmic/Endoplasmic reticulum Ca²⁺-ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation.

ABSTRACT: Liquid storage is the primary preservation method in the swine breeding industry because of its advantages over cryopreservation. Calcium (Ca²⁺), a key regulator of cell physiology, plays a crucial role during liquid preservation. Sarcoplasmic/Endoplasmic Reticulum Ca²⁺ ATPases (SERCA) belong to a family of P-type ATPases that regulate Ca²⁺ homeostasis within cells and have been previously described to play a function in the sperm of various mammalian species. Herein, we hypothesized that SERCA2 is present in pig sperm and is involved in the resilience of this cell to liquid preservation at 17 °C. For this purpose, sperm were incubated with different concentrations of thapsigargin (Thg; 0, 5, 25, and 50 µM) and stored at 17 °C for ten days. The presence and

SUBMITTER: Garriga F 

PROVIDER: S-EPMC11794610 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature