Biofabricated 3D Intestinal Models as an Alternative to Animal-Based Approaches for Drug Toxicity Assays.

ABSTRACT:

Background

The main challenge in new drug development is accurately predicting the human response in preclinical models.

Methods

In this study, we developed three different intestinal barrier models using advanced biofabrication techniques: (i) a manual model containing Caco-2 and HT-29 cells on a collagen bed, (ii) a manual model with a Caco-2/HT-29 layer on a HDFn-laden collagen layer, and (iii) a 3D bioprinted model incorporating both cellular layers. Each model was rigorously tested for its ability to simulate a functional intestinal membrane.

Results

All models successfully replicated the structural and functional aspects of the intestinal barrier. The 3D bioprinted intestinal model, however, demonstrated superior epithelial barrier integrity enhanced tight junct

SUBMITTER: Tofani LB 

PROVIDER: S-EPMC11794730 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature