Construction and evaluation of <i>Brassica rapa</i> orphan genes overexpression library.

ABSTRACT: Orphan genes (OGs) are crucial for species-specific characteristics and stress responses and are restricted to a specific taxon. However, their functions within particular species are poorly understood. Previous research identified OGs in Brassica rapa (BrOGs). In this study, the BrOGs overexpression (BrOGsOE) library in Arabidopsis thaliana was constructed. Approximately 128 unknown functional BrOGs were selected from Chinese cabbage and were overexpressed. The analysis focused on the phenotypes of leaf morphology and flowering time against phenotypic differences between Chinese cabbage and Arabidopsis. Interestingly, 72.66% of the transgenic lines showed distinctive phenotypic changes. Chinese cabbage-specific features, including cu

SUBMITTER: Jiang M 

PROVIDER: S-EPMC11794797 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature