Duplex qPCR for detecting and differentiating porcine epidemic diarrhea virus GI and GII subtypes.

ABSTRACT:

Introduction

Porcine epidemic diarrhea virus (PEDV) is a pathogen that causes a highly contagious intestinal disease in pigs, which causes significant economic losses to the pig industry worldwide. PCR is the most commonly used technique for PEDV diagnosis in practical clinics, however, reported works still suffer from shortcomings, for example, most of them cannot differentiate GI and GII subtypes, they suffer from low sensitivity, and some primer sequences are no longer able to match the mutant strains.

Methods

To address these issues, we conducted a comprehensive analysis by comparing the sequences of the PEDV S protein in the existing NCBI database with a recently isolated epidemic strain of PEDV, named SX0818-2022, of subtype GIIa from Shanxi, China. The conserved seque

SUBMITTER: Wang B 

PROVIDER: S-EPMC11794799 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature