DHARANI: A 3D Developing Human-Brain Atlas Resource to Advance Neuroscience Internationally Integrated Multimodal Imaging and High-Resolution Histology of the Second Trimester.

ABSTRACT: We introduce DHARANI, the first online platform with three-dimensional (3D) histological reconstructions of the developing human brain from 14 to 24 gestational weeks (GW) across the five fetal brains. DHARANI features 5132 Nissl, hematoxylin and eosin stained, 20 µm coronal and sagittal sections, postmortem MRI, and a neuroanatomical atlas with 466 annotated sections covering ∼500 brain structures. It is accessible online at https://brainportal.humanbrain.in/publicview/index.html. The 3D reconstruction enables a volumetric view of the fetal brain, allowing visualization in all three planes akin to MRI, previously unachievable with histological datasets from the fetal brain. This allowed qualitative assessment of the growth of brain regions and layers throughout the second trimester. "DHAR

SUBMITTER: Verma R 

PROVIDER: S-EPMC11794986 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature