A universal DNA microarray for rapid fish species authentication.

ABSTRACT: DNA microarrays are now used in fields such as gene expression analysis, pathogen/virus detection and identification of biomarkers. Although they have been used in the food sector for species identification, they detect a limited number of species and are thus less suited for fishery products due to the large variety of traded species. Here, the aim of this proof-of-principle study was to design a universal DNA microarray that should be able to distinguish all fish species by comparing hybridization signal patterns from samples with patterns obtained from reference specimens. A universal set of 100 DNA probes (based on the genetic marker genes 16S ribosomal RNA and cytochrome b) generated species-specific DNA probe patterns for all 86 analyzed fish specimens. This new screening meth

SUBMITTER: Bade P 

PROVIDER: S-EPMC11795077 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature