Enhanced small intestinal organoid-derived epithelial cell adhesion and growth in organ-on-a-chip devices.

ABSTRACT: Organ-on-a-chip devices are predominately made of the polymer polymethylsiloxane (PDMS), exhibiting several attractive properties e.g., transparency, gas permeability, and biocompatibility. However, the attachment of cells to this polymer has proven challenging, especially for delicate primary cells e.g., small intestinal organoid-derived epithelial cells. Hence, a need to functionalize and coat the surface has arisen to render it more hydrophilic and improve its ability to support cell adhesion and growth. While previous research has demonstrated some successful results in culturing primary cells, no comprehensive and comparative protocol has been proposed. Here, we provide a protocol for enhanced small intestinal organoid-derived epithelial cell adhesion and growth on PDMS

SUBMITTER: Quacquarelli F 

PROVIDER: S-EPMC11795259 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature