NCAPD3-mediated AKT activation regulates prostate cancer progression.

ABSTRACT: Despite therapeutic improvements in prostate cancer treatment, the recurrence and mortality rates are still high, and the underlying mechanisms still need further study. Non-SMC Condensin II Complex Subunit D3 (NCAPD3) is a subunit of condensin II complex, mainly involved in the mitotic chromosome condensation of cells. This study aimed to figure out the detailed mechanisms by which NCAPD3 contributed to prostate cancer development. Clinical samples and cell lines were used to measure the expression of genes by quantitative real-time RT-PCR (qRT-PCR), Western-blot assay (WB), immunohistochemistry (IHC), and immunofluorescence (IF). Chromatin immunoprecipitation quantitative PCR (ChIP-qPCR) and dual-luciferase reporter assays were examined to explore the interplays between molecules. CCK8,

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC11795278 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature