Multienzymatic Platform for Coupling a CCU Strategy to Waste Valorization: CO₂ from the Iron and Steel Industry and Crude Glycerol from Biodiesel Production.

ABSTRACT: Ongoing climate crisis demands the development of carbon capture and utilization (CCU) technologies that emphasize simplicity, eco-sustainability, and cost-effectiveness. Enzymatic CO₂ reduction emerges as an alternative to biotransforming this cheap raw material into high-value products under milder conditions. This work proposes a multienzymatic platform to reduce CO₂ to formate by formate dehydrogenase (FDH) and oxidize glycerol to dihydroxyacetone (DHA) by glycerol dehydrogenase (GlyDH), allowing for efficient cofactor regeneration. Through studies such as pH operating range, enzyme stability, FDH/GlyDH ratio, and reaction medium engineering to achieve optimal soluble CO₂ concentrations, the reaction with a gas mixture of 24% CO₂ yielded 5.7

SUBMITTER: Rodriguez SR 

PROVIDER: S-EPMC11795641 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature