HPLC-MS standardization and validation methods for determination of calactin content in dichloromethane fraction of <i>Calotropis gigantea</i> (L.) Dryand. (<i>Niu jiao gua</i>) stem bark and its application in prediction of anticancer activities.

ABSTRACT: Calactin, a doubly-linked cardenolide, is commonly found in Apocynaceae family including Calotropis gigantea (L.) Dryand. (Niu jiao gua, C. gigantea). This phytochemical has gained recognition for its potential as an anticancer agent and a marker for anticancer properties. Our previous reports demonstrated the remarkable anti-liver and anti-colon cancer activities both in vitro and in vivo of the dichloromethane fraction from the stem barks of C. gigantea (CGD). However, quantitative analysis of calactin content in CGD by using a high-performance liquid chromatography (HPLC) - ultraviolet (UV) method was limited. Therefore, this study aimed to develop more sensitive and reliable method for measurement of calactin in CGD by using a HPLC- electrospra

SUBMITTER: Kaewpaeng N 

PROVIDER: S-EPMC11795781 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature