Bioinformatics screening and clinical validation of CircRNA and related miRNA in male osteoporosis.

ABSTRACT:

Background

The pathogenesis of male osteoporosis (MOP) remains unclear, with the role of genetic factors attracting the attention of researchers. In the present study, we aimed to investigate critical circRNA biomarkers associated with male osteoporosis.

Methods

RNA-sequencing was performed to investigate the circRNA expression profiles between 3 men with osteoporosis and 3 with normal mass density. Then, shared mRNAs between host genes acquired in this present study and mRNAs acquired in previous study were identified to screen vital circRNAs associated with male osteoporosis. PPI networks of shared mRNAs were constructed and the hub genes in the PPI networks were identified with CytoHubba, a plugin in Cytoscape software (3.10.1). Finally, a ceRNA network of four circRNAs d

SUBMITTER: Li J 

PROVIDER: S-EPMC11796104 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature