Using RAD-seq to identify and differentiate the medicinal herb Scrophularia ningpoensis and its adulterants.

ABSTRACT:

Background

Scrophulariae Radix, the dried root of Scrophularia ningpoensis Hemsl., belongs to the Scrophulariaceae family and is known for its diverse pharmacological effects, such as antihypertensive, anti-inflammatory, and antitumor properties. It is common that the Scrophulariae Radix is often contaminated by various adulterants like S. buergeriana, S. kakudensis, and S. yoshimurae in its medicinal usage. However, traditional methods for identifying Chinese medicinal materials have been found to be insufficient in accurately distinguishing S. ningpoensis from the three adulterant species.

Results

In this study, RAD-seq technology was employed to sequence 27 individuals from the four species, yielding 55,250 high-quality SNP sites. Genetic differentiation analysis using th

SUBMITTER: Guo L 

PROVIDER: S-EPMC11796183 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature