Real-time multimodal imaging of daptomycin action on the cell wall of adherent Staphylococcus aureus.

ABSTRACT:

Objectives

This study investigated the efficacy of daptomycin against adherent Staphylococcus aureus (S. aureus), a common colonizer of medical devices that leads to severe infections. For the first time, we evaluated the bactericidal effects of daptomycin on S. aureus immediately after adhesion, mimicking early-stage contamination of biomaterials. Time-kill curve assay and confocal laser scanning microscopy (CLSM) were used to analyze the process dynamics. In addition, atomic force microscopy (AFM) and scanning electron microscopy (SEM) were employed to elucidate daptomycin-induced structural changes in the bacterial cell wall.

Results description

Daptomycin, at clinically relevant concentrations, rapidly eradicated adherent bacteria in the exponential growth phase, demonst

SUBMITTER: Canette A 

PROVIDER: S-EPMC11796211 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature