A gut-derived <i>Streptococcus salivarius</i> produces the novel nisin variant designated nisin G and inhibits <i>Fusobacterium nucleatum</i> in a model of the human distal colon microbiome.

ABSTRACT: Fusobacterium nucleatum is a human pathogen associated with intestinal conditions including colorectal cancer. Screening for gut-derived strains that exhibit anti-F. nucleatum activity in vitro revealed Streptococcus salivarius DPC6487 as a strain of interest. Whole-genome sequencing of S. salivarius DPC6487 identified a nisin operon with a novel structural variant designated nisin G. The structural nisin G peptide differs from the prototypical nisin A with respect to seven amino acids (Ile4Tyr, Ala15Val, Gly18Ala, Asn20His, Met21Leu, His27Asn, and His31Ile), including differences that have not previously been associated with a natural nisin variant. The nisin G gene cluster consists of nsgGEFABTCPRK with transposases encoded between the nis

SUBMITTER: Lawrence GW 

PROVIDER: S-EPMC11796361 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature