Phage-mediated virulence loss and antimicrobial susceptibility in carbapenem-resistant <i>Klebsiella pneumoniae</i>.

ABSTRACT: Bacteriophages, known for their ability to kill bacteria, are hampered in their effectiveness because bacteria are able to rapidly develop resistance, thereby posing a significant challenge for the efficacy of phage therapy. The impact of evolutionary trajectories on the long-term success of phage therapy remains largely unclear. Herein, we conducted evolutionary experiments, genomic analysis, and CRISPR-mediated gene editing, to illustrate the evolutionary trajectory occurring between phages and their hosts. Our results illustrate the ongoing "arms race" between a lytic phage and its host, a carbapenem-resistant Klebsiella pneumoniae clinical strain Kp2092, suggesting their respective evolutionary adaptations that shape the efficacy of phage therapy. Specifically, Kp2092 rapidly de

SUBMITTER: Yu Y 

PROVIDER: S-EPMC11796411 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature