Mapping <i>C. difficile</i> TcdB interactions with host cell-surface and intracellular factors using proximity-dependent biotinylation labeling.

ABSTRACT: Many bacterial toxins exert their cytotoxic effects by enzymatically inactivating one or more cytosolic targets in host cells. To reach their intracellular targets, these toxins possess functional domains or subdomains that interact with and exploit various host factors and biological processes. Despite great progress in identifying many of the key host factors involved in the uptake of toxins, significant knowledge gaps remain as to how partially characterized and newly discovered microbial toxins exploit host factors or processes to intoxicate target cells. Proximity-dependent biotinylation (e.g., BioID) is a powerful method to identify nearby host factors in living cells, offering the potential to identify host targets of microbial toxins. Here, we used BioID to interrogate proximal int

SUBMITTER: Ward JS 

PROVIDER: S-EPMC11796423 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature