Isolation of a tumor neoantigen specific CD8+ TCR from a skin biopsy of a vaccination site.

ABSTRACT: T cells that recognize tumor-specific mutations are crucial for cancer immunosurveillance and in adoptive transfer of TILs or transgenic-TCR T cell products. However, their challenging identification and isolation limits their use in clinical practice. Therefore, novel approaches to isolate tumor-specific T cells are needed. Here, we report the isolation of neoantigen-specific CD8⁺ T cells from a vaccination site of a metastatic breast cancer patient who received a personalized vaccine. Based on the somatic mutations, potential MHC binding epitopes were predicted, of which 17 were selected to generate a peptide vaccine. Cutaneous biopsies were processed after the fifth vaccination cycle to obtain infiltrating lymphocytes from the vaccination site (VILs). IFNγ ELISpot revealed re

SUBMITTER: Roberti MP 

PROVIDER: S-EPMC11796541 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature