A deficiency screen identifies genomic regions critical for sperm head-tail connection.

ABSTRACT: The Sperm Neck provides a stable connection between the sperm head and tail, which is critical for fertility in species with flagellated sperm. Within the Sperm Neck, the Head-Tail Coupling Apparatus serves as the critical link between the nucleus (head) and the axoneme (tail) via the centriole. To identify regions of the Drosophila melanogaster genome that contain genetic elements that influence Head-Tail Coupling Apparatus formation, we undertook a 2 part screen using the Drosophila Deficiency kit. For this screen, we utilized a sensitized genetic background that overexpresses the pericentriolar material regulatory protein Pericentrin-Like Protein. We had previously shown that Pericentrin-Like Protein overexpression disrupts the head-tail connection in some spermatids, but not to a degre

SUBMITTER: Galletta BJ 

PROVIDER: S-EPMC11797012 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature