A cost-effective, high-throughput, highly accurate genotyping method for outbred populations.

ABSTRACT: Affordable sequencing and genotyping methods are essential for large-scale genome-wide association studies. While genotyping microarrays and reference panels for imputation are available for human subjects, nonhuman model systems often lack such options. Our lab previously demonstrated an efficient and cost-effective method to genotype heterogeneous stock rats using double-digest genotyping by sequencing. However, low-coverage whole-genome sequencing offers an alternative method that has several advantages. Here, we describe a cost-effective, high-throughput, high-accuracy genotyping method for N/NIH heterogeneous stock rats that can use a combination of sequencing data previously generated by double-digest genotyping by sequencing and more recently generated by low-coverage whole-genome s

SUBMITTER: Chen D 

PROVIDER: S-EPMC11797033 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature