RNA elements required for the high efficiency of West Nile virus-induced ribosomal frameshifting.

ABSTRACT: West Nile virus (WNV) requires programmed -1 ribosomal frameshifting for translation of the viral genome. The efficiency of WNV frameshifting is among the highest known. However, it remains unclear why WNV exhibits such a high frameshifting efficiency. Here, we employed dual-luciferase reporter assays in multiple human cell lines to probe the RNA requirements for highly efficient frameshifting by the WNV genome. We find that both the sequence and structure of a predicted RNA pseudoknot downstream of the slippery sequence-the codons in the genome on which frameshifting occurs-are required for efficient frameshifting. We also show that multiple proposed RNA secondary structures downstream of the slippery sequence are inconsistent with efficient frameshifting. We also find that the base of th

SUBMITTER: Aleksashin NA 

PROVIDER: S-EPMC11797035 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature