Evaluation of Blood-CSF Barrier Transport by Quantitative Real Time Fluorescence Microscopy.

ABSTRACT:

Purpose

Transporters at the blood-cerebrospinal fluid (CSF) barrier (BCSFB) play active roles in removing drugs and toxins from the CSF. The goal of this study is to develop a fluorescence microscopy approach to quantitatively study the transepithelial transport processes at the murine BCSFB in real time.

Methods

Choroid plexus (CP) tissues were isolated from mouse lateral ventricles and incubated with anionic (fluorescein-methotrexate, 8-fluorescein-cAMP) or cationic (IDT307) fluorescent probes. The CSF-to-blood transport was imaged and quantified using compartmental segmentation and digital image analysis. Real time images were captured and analyzed to obtain kinetic information and identify the rate-limiting step. The effect of transporter inhibitors was also evaluated.

SUBMITTER: Sun A 

PROVIDER: S-EPMC11797611 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature