TMEM196 inhibits lung cancer metastasis by regulating the Wnt/β-catenin signaling pathway.

ABSTRACT:

Purpose

The TMEM196 protein, which comprises four membrane-spanning domains, belongs to the TMEM protein family. TMEM196 was identified as a candidate tumor suppressor gene in lung cancer. However, its role and mechanism in lung cancer metastasis remain unclear. Here, we study the role of TMEM196 in tumor metastasis to further verify the function in lung cancer.

Methods

In this study, we used qRT-PCR, western blot analysis and immunohistochemistry to examine the expression levels of TMEM196 and related proteins in lung cancer tissues and tumor cells. We utilized Transwell assays, xenograft nude mouse models, and TMEM196^-/- mouse models to evaluate the effects of TMEM196 on tumor invasion and metastasis. Finally, we used bioinformatics analysis and dual-luciferase

SUBMITTER: Chen J 

PROVIDER: S-EPMC11797904 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature