Lamp2 Deficiency Enhances Susceptibility to Oxidative Stress-Induced RPE Degeneration.

ABSTRACT:

Purpose

Autophagy and lysosomal degradation are vital processes that protect cells from oxidative stress. This study investigated the role of lysosome-associated membrane protein 2 (Lamp2), a lysosomal protein essential for autophagosome maturation and lysosome biogenesis, in maintaining retinal health under oxidative stress.

Methods

To induce oxidative stress, young Lamp2 knockout (KO) and wild-type mice received an intravenous injection of a low dose (10 mg/kg) of sodium iodate (NaIO3). We examined retinal histopathology and morphological changes in the RPE. The involvement of resident microglia or infiltrating macrophages was assessed using immunostaining, flow cytometry, and real-time PCR for chemokines and cytokines.

Results

After administering a low-dose NaIO3,

SUBMITTER: Wu G 

PROVIDER: S-EPMC11798339 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature