Incoherent color holography lattice light-sheet for subcellular imaging of dynamic structures.

ABSTRACT: The purpose of the article is to explore the need and advantages of using the incoherent color holography lattice light-sheet (ICHLLS) to provide multiwavelength quantitative monitoring of 3D cellular dynamics in live tissue to further understand complex functions of cells and cellular compartments. We have explored the use of incoherent color holography lattice light-sheet to investigate colocalization of fluorescent markers in live cells in intact tissue. Neuronal structures provide an attractive target for incoherent color holography lattice light-sheet. The cells show a complex architecture in 3D space in which signaling both between cells and within subcellular structures requires colocalization of proteins and lipids to function. During activity and over long periods it is important

SUBMITTER: Alford S 

PROVIDER: S-EPMC11798417 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature