Deterministic genetic barcoding for multiplexed behavioral and single-cell transcriptomic studies.

ABSTRACT: Advances in single-cell sequencing technologies have provided novel insights into the dynamics of gene expression and cellular heterogeneity within tissues and have enabled the construction of transcriptomic cell atlases. However, linking anatomical information to transcriptomic data and positively identifying the cell types that correspond to gene expression clusters in single-cell sequencing data sets remains a challenge. We describe a straightforward genetic barcoding approach that takes advantage of the powerful genetic tools in Drosophila to allow in vivo tagging of defined cell populations. This method, called Targeted Genetically-Encoded Multiplexing (TaG-EM), involves inserting a DNA barcode just upstream of the polyadenylation site in a Gal4-indu

SUBMITTER: Blanco Mendana J 

PROVIDER: S-EPMC11798575 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature