The Hedgehog-GLI1 Pathway Regulates Osteogenic Differentiation of Human Cervical Posterior Longitudinal Ligament Cells by BMP Signalling Pathway.

ABSTRACT: Cervical ossification of the posterior longitudinal ligament (OPLL) is an ectopic ossification disorder characterised by endochondral ossification. Its aetiology remains to be fully elucidated. This study aimed to clarify its pathogenesis through RNA sequencing of primary cells cultured from patients without cervical OPLL (control, PLL) and patients with cervical OPLL (disease, OPLL). We revealed for the first time the role of GLI1 within OPLL cells. Functional experiments indicated that GLI1, acting as a pivotal mediator between the upstream Hedgehog pathway and downstream BMP pathway, influences the pathogenesis of OPLL. The positive/negative effects on osteogenic differentiation following activation/inhibition of the Hedgehog pathway can be rescued by manipulating GLI1 expression. Overe

SUBMITTER: Xu W 

PROVIDER: S-EPMC11798735 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature