Single-cell sequencing reveals the features of adaptive immune responses in the liver of a mouse model of dengue fever.

ABSTRACT:

Background

Dengue fever, an acute insect-borne infectious disease caused by the dengue virus (DENV), poses a great challenge to global public health. Hepatic involvement is the most common complication of severe dengue and is closely related to the occurrence and development of disease. However, the features of adaptive immune responses associated with liver injury in severe dengue are not clear.

Methods

We used single-cell sequencing to examine the liver tissues of mild or severe dengue mice model to analyze the changes in immune response of T cells in the liver after dengue virus infection, and the immune interaction between macrophages and T cells. Flow cytometry was used to detect T cells and macrophages in mouse liver and blood to verify the single-cell sequencing resul

SUBMITTER: Yuan Y 

PROVIDER: S-EPMC11798745 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature