Identification and Application of the Heptad Repeat Domain in the CPR5 Protein for Enhancing Plant Immunity.

ABSTRACT: Plant resistance to pathogens can be significantly enhanced through genetic modification, thereby reducing the reliance on chemical pesticides. CONSTITUTIVE EXPRESSER OF PATHOGENESIS-RELATED GENES 5 (CPR5) serves as a key negative regulator of plant immunity. Here we explored the functional domains of the CPR5 protein with the goal of dampening its activity to bolster plant immunity. Using hexapeptide asparagine-alanine-alanine-isoleucine-arginine-serine (NAAIRS) linker-scanning analysis, we identified a heptad repeat domain (HRD) in the middle region of the CPR5 protein, which is highly conserved across the plant kingdom. The HRD is predicted to form an α-helix structure and acts as an interface for CPR5 dimerization. Intriguingly, overexpression of the HRD in Arabidopsis wild-type plants

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC11798864 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature