Nucleosome fibre topology guides transcription factor binding to enhancers.

ABSTRACT: Cellular identity requires the concerted action of multiple transcription factors (TFs) bound together to enhancers of cell-type-specific genes. Despite TFs recognizing specific DNA motifs within accessible chromatin, this information is insufficient to explain how TFs select enhancers¹. Here we compared four different TF combinations that induce different cell states, analysing TF genome occupancy, chromatin accessibility, nucleosome positioning and 3D genome organization at the nucleosome resolution. We show that motif recognition on mononucleosomes can decipher only the individual binding of TFs. When bound together, TFs act cooperatively or competitively to target nucleosome arrays with defined 3D organization, displaying motifs in particular patterns. In one combination, mo

SUBMITTER: O'Dwyer MR 

PROVIDER: S-EPMC11798873 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature