Single-cell RNA sequencing and AlphaFold 3 insights into cytokine signaling and its role in uveal melanoma.

ABSTRACT:

Background

Uveal melanoma (UVM) is a form of eye cancer with a poor prognosis, particularly in metastatic patients. This study aimed to elucidate the cellular heterogeneity within UVM and identify prognostic biomarkers.

Methods

We performed single-cell RNA sequencing (scRNA-seq) on primary and metastatic UVM samples. A UVM-specific gene signature was constructed using LASSO regression and validated via ROC curve analysis in the TCGA-UVM and GSE84976 cohorts. AlphaFold 3 was used to predict the 3D structures of key proteins. T-cell populations were analyzed using pseudotime trajectory mapping and interaction network visualization. CRISPR-Cas9 screening analysis was conducted to identify hub genes and cytokine pathways that may serve as therapeutic targets. Additionally, we co

SUBMITTER: Sun H 

PROVIDER: S-EPMC11798937 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature