Induced protein expression in Leptospira spp. and its application to CRISPR/Cas9 mutant generation.

ABSTRACT: Expanding the genetic toolkit for Leptospira spp. is a crucial step toward advancing our understanding of the biology and virulence of these atypical bacteria. Pathogenic Leptospira are responsible for over 1 million human leptospirosis cases annually and significantly impact domestic animals. Bovine leptospirosis causes substantial financial losses due to abortion, stillbirths, and suboptimal reproductive performance. The advent of the CRISPR/Cas9 system has marked a turning point in genetic manipulation, with applications across multiple Leptospira species. However, incorporating controlled protein expression into existing genetic tools could further expand their utility. We developed and demonstrated the functionality of IPTG-inducible heterologous protein expression in Leptospira spp.

SUBMITTER: Fernandes LGV 

PROVIDER: S-EPMC11799391 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature