Peripheral membrane protein endophilin B1 probes, perturbs and permeabilizes lipid bilayers.

ABSTRACT: Bin/Amphiphysin/Rvs167 (BAR) domain containing proteins are peripheral membrane proteins that regulate intracellular membrane curvature. BAR protein endophilin B1 plays a key role in multiple cellular processes critical for oncogenesis, including autophagy and apoptosis. Amphipathic regions in endophilin B1 drive membrane association and tubulation through membrane scaffolding. Our understanding of exactly how BAR proteins like endophilin B1 promote highly diverse intracellular membrane remodeling events in the cell is severely limited due to lack of high-resolution structural information. Here we present the highest resolution cryo-EM structure of a BAR protein to date and the first structures of a BAR protein bound to a lipid bicelle. Using neural networks, we can effectively sort partic

SUBMITTER: Thorlacius A 

PROVIDER: S-EPMC11799418 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature