Enhanced replication of a hepatitis A virus vaccine strain via adaptation in Vero cells.

ABSTRACT:

Purpose

Hepatitis A virus (HAV) production has been limited by its slow replication rate and reliance on diploid cell lines like MRC-5, which present challenges in scalability, passage limitations, and serum-free culture conditions. This study aimed to develop an HAV vaccine strain with enhanced replication capacity.

Materials and methods

We generated a reverse genetically modified HAV vaccine strain (RG-HAV) and adapted it to Vero cells through sequential culturing. Replication rates of RG-HAV and a commercially used strain, HM-175, were compared in Vero and MRC-5 cells. Nucleotide sequences, including coding and non-coding regions like the internal ribosomal entry site (IRES), were analyzed. Structural assessments included 3-dimensional modeling of IRES and relative codon

SUBMITTER: Seo SH 

PROVIDER: S-EPMC11799578 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature