Discovery of novel vaccine candidates based on the immunogenic epitopes derived from Toxoplasma membrane proteins.

ABSTRACT:

Purpose

Due to the widespread distribution and importance of Toxoplasma gondii infection as a parasitic zoonosis, multi-epitope vaccine design was implemented using a set of immunodominant epitopes screened out of a wide scope of membrane proteins.

Materials and methods

On this basis, 5 vaccine candidates were created using linkers ([GGGGS]₂, KK, AAY, GPGPG, GDGDG, EAAAK) and adjuvants (RS-09 peptide, Mycobacterium tuberculosis resuscitation-promoting factor E [RpfE] and 50S ribosomal protein, human interferon [IFN]-γ).

Results

Polytopes with RS-09 alone (Toxo-App) and with IFN-γ (Toxo-Apfn), and one with 50S ribosomal protein (Toxo-Ribos) showed the highest immunogenicity during in silico prediction, and their 3-dimensional structure w

SUBMITTER: Hosseini SA 

PROVIDER: S-EPMC11799584 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature